MPN in Water Testing – EC BlueQuant

For fast and easy quantification of coliform bacteria and E.coli as MPN in Water testing (Most Probable Number), e.g. according to DIN EN ISO 9308-2:2014-06. Optimised for use with EC Blue 100 and 100P. Detection limit: 1 cfu/100 ml for coliforms and E. coli.

The unique, innovative design of the EC BlueQuant/MPN allows rapid and reliable processing of MPN tests – simple to operate and without any additional equipment, except an incubator. The method is based on the standardised MPN method that uses at least three different dilution levels in increments of 10 and 5 compartments per dilution. Testing with the EC BlueQuant/MPN does not require a series of dilutions. When pouring the mixture, due to the special layout, the compartments are filled in such a way as to ensure correct serial dilution. After incubating the sample for 24 hours at about 35 °C, the result can be read off the device and converted to cfu/ml using ISO standard tables (e.g. DIN EN ISO 9308-2:2014-06).

Presence of E. coli can easily be detected by the occurrence of fluorescence under UV light (see EC-Blue Mini UV lamp Art. No. KK49.1). Approximately 95 % of all E. coli strains produce the enzyme ß-glucuronidase, which reacts with an enzyme substrate contained in EC Blue to release a fluorescent dye.