Membrane Filter Cellulose Acetate 0.65 μm

0.65 µm cellulose acetate (CA) membrane filters are hydrophilic, low-protein-binding microfiltration membranes used for particle reduction, clarification, and filtration of aqueous/proteinaceous solutions where high recovery and higher flow rates are needed (compared with tighter 0.45 or 0.22 µm pores

Key Characteristics of 0.65 µm CA

• Pore size: Nominal 0.65 µm — retains larger particles, yeast, molds, and some larger organisms, but is not a sterilizing grade for typical bacteria (use ≤0.45 µm or 0.22 µm for that).
• Flow rate: Higher than 0.45 µm (examples: ~70.9 mL/min/cm² at 10 psi for some CA vs. ~54.7 for 0.45 µm).
• Bubble point: Typically around 18 psi (varies by manufacturer).
• Protein binding: Extremely low (one of the lowest among common membranes), ideal for minimizing sample loss.
• Other: Autoclavable/steam sterilizable (up to ~135°C), hydrophilic, good dimensional strength (often polyester-supported), low extractables

Typical Applications

• Prefiltration and particle reduction: Clarification of water, culture media, buffers, or solutions containing proteins, enzymes, antibiotics, or other adsorbable ingredients — reduces particle load while minimizing non-specific adsorption losses.
• Biological/clinical samples: Filtration of proteinaceous solutions, biological fluids, and tissue-culture media where maximum recovery of proteins/enzymes is critical and sterilizing filtration is not required.
• General laboratory use: Aqueous solution filtration, HPLC sample/mobile-phase prep (when coarser filtration suffices), diagnostic work, and situations needing higher throughput than 0.45 µm.
• Food/beverage and pharmaceutical: Clarification steps in juices, dairy, or other liquids; prefiltration before tighter membranes.
• Microbiology-related: While pure CA is less common than mixed cellulose esters (MCE) for routine colony counting, 0.65 µm pores are used for yeast/mold retention or recovery of certain larger organisms; CA may be chosen when low protein binding is advantageous.